Eva

This past semester I studied abroad in Italy and I did the home-stay option with an amazing italian lady. We became really great friends and she invited me to come to her 40th birthday party which will be held in Berlin in October. During my time abroad I did not get along well with any of the other students in my program, but my italian lady friend was always there to cheer me up and try to teach me new things. Anytime she would go somewhere (even just coffee with her friends) she would invite me to come along and make me feel welcome. She even came to my end of the year student showcase to view my work. So long story short...I want to go to Germany in October to support my friend for her birthday. The issue is that it is expensive to travel to Europe twice in the same year :( but I feel if I visit her later then the significance will not be the same (because apparently 40th birthdays are really big in Europe)

In terms of my financial situation, I currently have to pay off $5500 in student loans that I took out to study abroad in the first place. Travel fees for this new trip will be around $2000. I have a steady job and I am slowly paying off the loans, right now I can defer the payments because I am still in school. I would pay for the plane ticket with my credit card and slowly pay it off.

My boyfriend and family are against me going but ultimately it is my decision. I feel like going to Europe isn't the most practical choice but it is possible.

Here is the background info and questions:

You just identified a yeast temperature sensitive mutant (scc1ts) that appears to have defects in sister chromatid separation. To assay directly for sister chromatid cohesion, you insert a tandem array of 336 short DNA sequences to which the bacterial protein X can bind tightly, adjacent to the centromere of chromosome V. When you express GFP tagged X (GFP-X) in these cells, you see a bright dot of fluorescence due to X-GFP binding to the DNA repeats mentioned above. To test for the effects of mutant SCC1 on sister chromatid cohesion, you isolate unbudded cells from wild-type and scc1ts cells that were grown at 25oC and shift them to 37oC for various times. Representative examples of small-budded cells in S phase and large-budded cells that have passed the metaphase-anaphase transition are shown for both strains shifted at 37oC in the figure below. Hint: consider how temperature sensitive mutations work.

1a. Do sister chromatids in wild-type adhere to each other normally during S phase and separate normally during mitosis? How can you tell?

1b. Do sister chromatids in scc1ts cells adhere normally in S phase and separate normally during mitosis? How can you tell?

1c. In the large budded cells from the scc1ts strain, why do both sister chromatids remain in one cell?

Even if you could just answer part of the question it would realy help me out. I want to get a good grade on this but I don't really understand this stuff. Thank you so much in advance!

The DF508 mutation in the Cystic Fibrosis Transmembrane conductance Regulator (CFTR) affects protein folding but not the channel function of the translated peptide. It is known that in patients carrying this mutation, the channel is made but does not localize properly. What do you suppose happens with the misfolded CFTR protein? Can you propose a therapeutic approach based on your answer to the previous question?

Soluble proteins that need to reside in the ER have a retrieval 
signal, the amino acids KDEL. This signal is detected by a KDEL-receptor protein, which 
is a membrane protein that binds the KDEL signal on proteins that have escaped to the 
Golgi. The receptor packages the proteins into vesicles for transport back to the ER.


A. What would be the fate of an ER resident protein that had its KDEL signal removed?


B. Do you think the KDEL-receptor is more concentrated in the ER or the Golgi? Explain 
briefly


C. What coat protein will be on the vesicles for return to the ER?


D. Does the KDEL-receptor itself need an ER retrieval signal? Explain briefly.

So I'm an intern at the zoo and one of the projects that I have to do is make a creative toy/enrichment item (something they will find entertaining) to give to one of the animals I work with. I really want to make an impressive toy but so far my mind has been drawing a blank about what to give them. Here's a little bit of info about each of the animals I work with:

Polar bear: her current toys are thick plastic blocks (think giant ice cubes), balls, burlap sacks, thick ropes, and thick plastic rings. She really likes peanut butter, honey, yams, and obviously meat. Sometime we will give her pine cones covered in honey and she loves them. She has a large pool in her exhibit and also a large grassy area. There really are no limitations in terms of the material the toy I can make her. During christmas she gets christmas trees to tear up. The toy can be something intricate to hide food in that she can mess with.

Lions: they really like soccer sized balls. They also have large pieces of wood to chew on. They can't have anything made of thin plastic or fabric. I was thinking maybe something with feathers to attract them but I couldn't think of what.

Baboons: they love ripping open boxes! Everyday their food is hidden in boxes for them to find and open. I was hoping to make some sort of intricate puzzle box for them to figure out but I'm open to suggestions as to what the design should be. They also like picking things out of holes, in their exhibit there is a giant tree stump with holes drilled into it and they like picking food out of it. Basically they would enjoy any type of toy a five year old or younger would enjoy.

Anteater: they like ripping things apart with their long claws. They don't really have any actual toys besides pieces of wood that they tear apart. They also like oranges because they can rip them apart too.

Andean bears: pretty much will get into anything. They have some sticks and pastic balls to play with but I think they could really use more interesting toys.

Tapir: doesn't have any toys. He likes veggies and swimming in his exhibit pool. Can nudge around at things with his nose pretty well.

Capybara: Not really sure what they guys might like. They are pretty shy. I know they like the water though.

Any suggestions for any or all of these animls would be great. If I like your idea I might just make it and be able to give it to them, and then I can let you know what they do with it. Thank you!!!

can someone please explain why benzene rings require such a strong electrophile for a substitution reaction to occur? Thank you!

H2C=CHCH3, HF, H3CCH3, HC=CCH3 (actually triple bond instead of the double bond), H3CNH2

which movie do you think is better: Click, Yes Man, or Stranger than Fiction? these are all similar movies but which movie is the best and explain why!?!

Assume you are studying a genetic defect wherein troponin binds to sodium instead of calcium ions. Would muscle contraction function properly in this individual? Why or why not?

I get so jealous of my boyfriend's two best friends. One is a guy and one is a girl. Whenever they come over to hang out at my boyfriend's house, his family is overly nice to them. They actually have conversations with them, joke around with them, give them hugs, say that they miss seeing them. But when i come over they won't even say hi or bye to me unless i say it first, and they just overall don't seem to like having me around too much. i love my boyfriend, and i admit we do see each other like everyday. We have been going out for 8 months. But i hate hanging out with his friends at his house and seeing the huge difference between the way his family treats me and his friends. i feel like i'm invisible when i'm with them. i admit i am kinda shy but i want his family to like me so i'm willing to do what it takes to make them like me better. i find myself being really jealous of his friends but i just can't help it. what can i do?

I'm trying to find the acceleration but i have no idea what mg stands for in this equation. please help asap!

i need information about the characteristics of the greeks described in the Iliad such as religious practices, values, and attitudes toward power. if you have any quotes that would be great too! please help! i need answers asap!

An author is paid $2 million to write a book and the marginal costs of publishing the book is a constant $10 per book. i know the quantity demanded and the price, how do i determine total costs?

Price Quantity Demanded

100 0

90 100,000

80 200,000

70 300,000

Can someone please show me the steps to solve these problems?

Find a power function that passes through (4, -5) and (6, -2).

Find the inverse function of f(x) = (ln(x)+5)/(2ln(x)+6) Hint: first rewrite f(x)

At the same time that a car passes through an intersection traveling east at 30 miles/hour, a truck traveling south towards the intersection at 40 miles/hour is 50 miles from the intersection. Determine the time at which the car and truck are closest to each other.

can someone please help me solve this problem and show me the steps they used to do it? thanks soooo much!!!!

i am a newly trained swim lesson instructor and my lessons start tomorrow! my supervisor chose me to teach the parent/infant classes and i am seriously freaking out about it! i am a really soft spoken person and i really dont like "performing/presenting" in front of ppl. i could have handled the kids just not the parents. i want know what "songs" i could sing and the drills that i could teach along with them. any advice at all would be greatly appreciated! thanx!