Need help with biochem homework.?
"You process your patient s BUN sample and determine its % Transmittance to be 17.0. What is the concentration of BUN in your patient s sample? (Note: The patient is a male in his mid-forties)
"You process your patient s BUN sample and determine its % Transmittance to be 17.0. What is the concentration of BUN in your patient s sample? (Note: The patient is a male in his mid-forties)
hcbiochem
There has to be more information available to you for this problem. You will need to know the extinction coefficient for this assay.
First, convert the %T into an absorbance using the equation: Absorbance = 2-log%T
Then, use Beer's Law to calculate a concentration:
Absorbance = e b c where
e = extinction coefficient
b = path length (normally, 1 cm)
c = concentration in units determined by the extinction coefficient