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Meghan
Meghan asked in Science & MathematicsChemistry · 2 years ago

Need help with biochem homework.?

"You process your patient s BUN sample and determine its % Transmittance to be 17.0. What is the concentration of BUN in your patient s sample? (Note: The patient is a male in his mid-forties)

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  • hcbiochem
    Lv 7
    2 years ago

    There has to be more information available to you for this problem. You will need to know the extinction coefficient for this assay.

    First, convert the %T into an absorbance using the equation: Absorbance = 2-log%T

    Then, use Beer's Law to calculate a concentration:

    Absorbance = e b c where

    e = extinction coefficient

    b = path length (normally, 1 cm)

    c = concentration in units determined by the extinction coefficient

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