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How to tell what microorganism ferments indole?
1 Answer
- Anonymous1 decade agoFavorite Answer
If you have an unknown sample, you would have to incubate in SIM medium (stands for sulfur, indole, motility). Then the following day add Kovac's reagent and agitate. A cherry-red ring around the top of your test tube is a positive reaction, indicating indole production. The SIM test is only performed to differentiate the family Enterobacteriaciae, which are all Gram - rods. If you don't have a Gram - rod, then this test is of little to no value to you.
If you just want to know if a specific species produces indole, consult Bergey's manual (the Bible of microbiology!).