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Southern Blotting question?
In most cases there are two major reasons why the DNA must be denatured, either during or after blotting onto the membrane.
What are they?
2 Answers
- Anonymous1 decade agoFavorite Answer
A Southern blot is a method routinely used in molecular biology to check for the presence of a DNA sequence in a DNA sample. Southern blotting combines agarose gel electrophoresis for size separation of DNA with methods to transfer the size-separated DNA to a filter membrane for probe hybridization. The method is named after its inventor, the British biologist Edwin Southern.[1] Other blotting methods (i.e., western blot, northern blot, southwestern blot) that employ similar principles, but using RNA or protein, have later been named in reference to Southern's name. As the technique was eponymously named, Southern blot should be capitalized, whereas northern and western blots should not.
Source(s): http://en.wikipedia.org/wiki/Southern_blot - ?Lv 45 years ago
They are both electrophoresis procedures; Northern blot is used to analyze RNA while Southern blot is for DNA. Another common one is Western blot, which is used to analyze a protein sample.