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BIOCHEMISTRY HELP!!!!!!!!?
Ok, so I have a few questions from my biochem that I don't understand at all and the teacher doesn't help very much in the slightest.
I have an exam tomorrow morning and I'm freaking out.
Best Answer goes to whoever can explain it in the simplest yet most thorough way possible.
1.) Given 0.1 M solutions of acetic acid and sodium acetate, describe the preparation of a 0.1 M M acetate buffer at a pH of 5.4
2.) Hydrochloric acid is a significant component of gastric juice. What is the concentration of chloride ion in gastric juice if pH = 1.2?
3.) From the Pka for lactic acid given in Table 2.4 (3.86), calculate the concentratrion of lactate in blood plasma (pH = 7.4) if the concentration of lactic acid is 1.5 microM.
2 Answers
- deadfishfactoryLv 710 years agoFavorite Answer
1 and #3 requires you to use the Henderson-Hasselbalch equation, while #2 requires you to use the pH equation and the basic knowledge of mass balance/stoichiometry. I will do one and hopefully you can figure out the rest:
1)
pH = pKa + log[A-/HA]
pKa = 4.74
[A-] = sodium acetate
[HA] = acetic acid
5.4 = 4.7 = log[A-/HA]
[A-]/[HA] = 5.0
The ratio above calls for 5x the amount of [A-] per [HA] for it to be buffered at a pH = 5.4.
Without knowing how much volume you are making of the buffer, I can't give any quantitative answers.
- Anonymous4 years ago
Doen't Qur'an inspire to go out and look on the international, the creations, the animal, the flora, the factors etc? then, how can analyzing biochemistry be Haram? merely wash najees after practicals