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whendovescry
whendovescry asked in Science & MathematicsChemistry · 7 years ago

Finding grams from concentration of protein?

If I have 8.45 nM of enzyme, and I added it to a 100 microliter reaction, how many micrograms did I add?

The molecular mass of the enzyme is 59143

1 Answer

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  • hcbiochem
    Lv 7
    7 years ago

    8.45 nM = 8.45X10^-9 mol/L

    100 uL = 1X10^-4 L

    So, 8.45 X10^-9 mol/L X 1X10^-4 L = 8.45X10^-13 mol of enzyme

    Mass of enzyme = 8.45X10^-13 mol X 59143 g/mol = 5X10^-8 g enzyme

    5X10^-8 g enzyme X 1X10^6 micrograms/g = 0.050 micrograms of the enzyme

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