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Limit of detection question?
You want to determine the amount of cholesterol in the blood of a newborn infant. You know it will be around 5 mM. The detection limit of the method you are going to use is about 20 nm and you can only take about 20 microliters of blood. Will this be enough?
Thank you
1 Answer
- Peter HLv 76 years ago
It would help to get the units right. The normal blood cholesterol CONCENTRATION is 5mM/l, not 5mM. Now your problem is purely one of arithmetic. If your detection method can detect 20nM MASS in 20 micrometers VOLUME, what does that represent as a minimum detectable concentration, bearing in mind that concentration = mass/volume?
