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Ralph Carvil
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Organic chemistry help?
Why do we use a dye (Bradford reagent) to help determine the protein concentration?
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- Simonizer1218Lv 74 years ago
The dye (Bradford reagent) reacts with the protein to produce a color (blue) which is then readily detected by a colorimeter/spectrophotometer, and can be easily quantified. This allows one to measure the amount of protein in an unknown sample by comparing it to a series of standard protein samples.
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