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Lisa B
Problem with 1% agarose gel?
After running electrophoresis on 1% agarose gels in my lab, bubbles appear within the gel. The gels appear perfectly fine during and after pouring, and even after adding the samples. It's only after they've been run under the electrical current that the bubbles appear inside. Why might this be happening, and how might we avoid this?
1 AnswerBiology7 years agoHow do I make a 0.015% solution of...?
I need to make 5 mL of a 0.015% (w/v) solution of resazurin. How many milligrams of resazurin do I need?
1 AnswerChemistry8 years agoWhen does the Naruto movie Road to Ninja come out subbed?
It just came out in theaters in Japan a little while ago, but as I don't live in Japan or speak fluent Japanese, I have to wait for subtitles on the internet. WHEN DOES A FULL SUBBED VERSION COME OUT?!
5 AnswersMovies9 years agoHELP! Spider mite problem on plants?
I have a bad two-spotted spider mite infestation on my strawberries and cilantro (indoor potted), that I think might be starting to spread to a few of my other plants. How do I get rid of them?! Do I have to buy some sort of special soap, or can diluted dish soap solution work?
4 AnswersGarden & Landscape9 years agoRenewing driver's license?
So I just recently turned 21, and I was all excited to get a new over-21 license, because I don't like my under-21 style license (the orientation is different for over 21 and under 21, and I hate my picture from when I was 16). I thought my license expired on my 21st birthday, too. But I checked, and the expiration date says it doesn't expire for 2 more years. Can I still get a new one?
Also, for reference... I live in Massachusetts.
5 AnswersInsurance & Registration9 years agoIntro physics lab question on lenses?
Does anyone know if it's possible to find the focal length of a diverging lens experimentally (by combining with a converging lens and measuring the distances of the image vs the object) if the focal length of the converging lens is greater than the absolute value of the diverging focal length???
Exact procedure from manual on experimentally finding the value of a diverging lens:
"Place the converging and diverging lenses together as close as possible. Find the focal length f(combined) of the combination using a distant object and a screen. Let f(c) and f(d) be the focal lengths of the converging and diverging lenses respectively. Then use 1/f(combined) = 1/f(c) + 1/f(d) and the previously measured, averaged value of fc to find the focal length of the diverging lens f(d)."
***Question: Would this experiment be possible if f(c) > |f(d)|?***
Extra: In the lab, f(c) was 10cm and f(d) was -15cm.
2 AnswersPhysics9 years agoOdd bruise/lump in upper thigh?
A few weeks ago I noticed this odd little bruise about halfway along my left upper inner thigh. I didn't remember getting injured, but I have a tendency to get hurt without noticing, so I didn't think much of it besides keeping an eye on it. It's still there, and I notice that the discoloration seems to be on top of a hard lump under the skin. The discoloration is slightly oval, approximately 1 cm lengthwise, and a very light bruise color. It does not hurt, even when I press on it, and is otherwise unnoticeable. I'm a little worried, and possibly plan to see a doctor about it. However, I would like a few opinions on what it might be.
Other health information: I'm female, 21 years old, 5'5" and approximately 135 pounds. I'm otherwise fairly healthy.
2 AnswersCancer9 years agoSkyrim: Blood on the Ice quest glitch?
So I've started the quest, but after I talked to the witnesses, I can't talk to the guard. Like, I try, and he just goes "Wait... I know you." Nothing about the quest. How do I fix this? Or are there alternatives?
1 AnswerPC9 years agoHelp! Trying to come up with a fake disease, need a name?
For a microbiology project, I need to design my own microbe. I have it pretty much planned out, but I need a name for the disease it causes. Here's some info on it:
*Viral
*Transmitted through inhalation of droplets or blood transfer
*Moves through the lungs into the bloodstream where it then moves through the blood-brain barrier to the brain
*Symptoms:
Stage 1: Coughing, sputum, difficulty breathing, bronchitis
Stage 2: Fever, anemia, low blood pressure, rapid heart rate, rapid breathing
Stage 3: Confusion, headache, dizziness, cranial swelling/meningitis, fatigue
Stage 4: Death
I'm really bad with names... If you could help me come up with a name for this disease, that would be great.
4 AnswersHomework Help9 years agowho was that guy on the recent Colbert Report who was obsessed with finding penises in art?
Like, that old guy on the 12/6 show that thought the airport had phallic symbols in all the art. What's his name?
2 AnswersMedia & Journalism9 years agoGood relaxation/meditation music?
I'm interested in buying some CDs of relaxation music, like nature sounds, wind chimes, piano... Anyone know any particularly good titles I can get easily, or what sort of stores might sell some good stuff for cheap?
8 AnswersReligion & Spirituality10 years agoAre my eggs still good?
I bought fresh eggs yesterday, expiration date in December, and later that night the power went out for about 9 hours. The fridge was not opened at all during that period. Are my eggs still edible?
3 AnswersOther - Food & Drink10 years agoA 0.69 kg bullfrog is on a log tilted 24 deg above horizontal.?
How large is the normal force of the log on the bullfrog, and how do I calculate this?
1 AnswerPhysics10 years agoDid I over-react in this situation?
I went to a Burton's Grill with my family for dinner. We all ordered steak, and we were eating when my dad noticed something under my mom's steak. I pulled it out, and it was a plastic cup lid, like they have at fast-food places. I got really, really grossed out and almost puked, which resulted in me having a panic attack (I'm anxiety-prone), and had to go to the bathroom to calm down. After that I refused to eat any more, even though my parents kept eating. Even my mom! But they think I over reacted, because it wasn't even my steak, and it "wasn't the worst thing they've had happen." What do you think?
Note: The manager did apologize and give the steak free.
3 AnswersMental Health10 years agoHow do makeup, accessories, clothing, and other props affect identity?
How do they affect the way we present ourselves to the world? How can these things shape or create an alter ego, personality, or identity?
1 AnswerHomework Help1 decade agoWhy do anaerobic soil bacteria stink?
In my microbiology lab, we cultivated plates of CDC blood agar with soil inoculate and incubated them anaerobically. When we observed them later, they stank really, really badly. Why did the anaerobic soil bacteria stink?
2 AnswersBiology1 decade agoWhere can I find lyrics for "Again" by no3b?
I really want the lyrics for the song "Again" by no3b. It's the ending theme song for the anime Beelzebub, and they just came out with the full song! I love the song, and want to be able to sing along. Does anyone know where I can find the lyrics, or how long it will take for them to come out if they haven't already?
2 AnswersLyrics1 decade agoWhat do you think of this about God?
Is God willing to prevent evil, but not able? Then he is not omnipotent.
Is He able, but not willing? Then he is malevolent.
Is He both able and willing? Then whence cometh evil?
Is He neither willing nor able? Then why call him God?
8 AnswersReligion & Spirituality1 decade agoLate period; should I see a doctor?
My period seems to be rather late. I was expecting it around January 18, and I still haven't gotten it. I'm not even experiencing the normal symptoms I get right before a period, i.e. cramps, back pain, and sore breasts. I don't keep written track of my period start and end, and sometimes it's a little irregular, but I've never been so off in my estimate before. All I can remember for sure is that my last period was some time before winter break ended and I went back to school on Jan 12. I am a virgin, and I have never even been near a naked penis or its secretions, much less within the time since my last period. I'm slightly anemic, but that's never really been a problem before. Could there be something wrong with my body, and I should see a doctor soon? Or am I just overreacting, and should give it another week or so before taking more advanced measures?
2 AnswersWomen's Health1 decade ago