Yahoo Answers is shutting down on May 4th, 2021 (Eastern Time) and beginning April 20th, 2021 (Eastern Time) the Yahoo Answers website will be in read-only mode. There will be no changes to other Yahoo properties or services, or your Yahoo account. You can find more information about the Yahoo Answers shutdown and how to download your data on this help page.

Trending News

Promoted
starla_o0
Lv 4
starla_o0 asked in Science & MathematicsBiology · 1 decade ago

Indirect immunofluorescence?

I performed indirect immunfluorescence of cells which had gone under apoptosis. The dyes/stains were DAPI and FITC. What should I be looking for when looking at the slides that indicates apoptosis?

3 Answers

Relevance
  • Alan V
    1 decade ago
    Favorite Answer

    DAPI will stain and allow you to visualize your DNA.

    During apoptosis, chromatin undegoes irreversible condensation in which you will see compact patches against the nuclear envelope. The nuclear envelope and DNA will then fragment into little chunks. THese processes are called pyknosis and karyorrhexis, respectively.

    Therefore, normal cells would show a full and smooth opaque nucleus and possibly a few cells in mitosis, whereas cells undergoing apoptosis would show condensed and fragmented chromatin.

  • endac
    1 decade ago

    Come on its 01.20 in the morning over here. Ask it again in the morning.

  • Anonymous
    1 decade ago

    fish fingers

Still have questions? Get your answers by asking now.