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Explain how to determine base-change specificity of a mutagen?
Its my last problem, and I'm confused. If you can, please supply websites that might help explain this better.
thanks
1 Answer
- Anonymous1 decade agoFavorite Answer
In order to determine the specificity of the alteration, you examine the amino acid changes that take place after mutagenesis.
A strong experimental approach to determining the mode of action of a mutagen is to examine the revertability of the products of one mutation by other mutagens (called reversion analysis), since specific base-pair substitutions can be inferred from these reversion events. A single base change, or point mutation, can in theory be distinguished by its reversion behaviors in response to specific mutations.
http://www.uiw.edu/genetics/chap_16.htm
Reversion analysis -