GMO lab in biology.....?

A very simple way is to use x-gal. Your plasmid would have the beta-galactosidase gene (which cuts x-gal in half, which makes the colony blue).

Then you do a digest and insert your gene into the beta0galactosidase gene. When the bacteria try to make the beta-galactosidase enzyme, it won't be able to make it because the gene was cut. See digram below: Beta-gal = beta-galactosidase gene

Insert= fruit fly gene

Plasmid......Beta-Galactosidase gene.....plasmid

-------------|-------------------------------------------|-----------------------------

You will get Blue Colonies

__________________________

Plasmid....beta-gal.....Insert...............Beta-gal.........plasmid

-------------|--------------|-----------------------|---------------|----------------------

You will get White Colonies

You will see blue and white colonies on your agar plate.

The white colonies have the fruit fly gene. Pick that colony and grow it up.

White colonies mean that the transformation was successful and that you successfully inserted your fruit fly gene into your plasmid.

good luck.

very often the fluorescent genes are located on a plasmid that also include a gene for ampicillin (or another antibiotic) resistance. To see if the transformation was successful, you could grown the E. coli in an agar dish containing ampicillin. if you observe colonies growing, then you know the gene was picked up by the bactera. it requires a little more work, but it is very helpful.

You could dna test and look for the specific or matching genes