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biology 1 help?! please?!?
Okay so I just did a lab and am still confused. We did a gel electrophoresis lab with 4 samples
1. Loon yodel gene
2. Bacterial plasmid Yodel gene treated with MwoI
3. Bacterial plasmid with Yodel gene treated with EcoRI
4. Linearized bacterial plasmid by itself
Which 2 samples would show up the same/equal length in the gel and out the 2 remaining which would be larger and smaller? Thank you!!
2 Answers
- Mark S, JPAALv 77 years ago
OK--the plasmid by itself will be one band. The two restriction digests *could* be one band, of the same length, if there is only one MwoI and one EcoRI site in the plasmid. Otherwise they will form several smaller bands. The gene itself will almost certainly be larger than the linearlized or digested plasmid samples.
- Sir Mart AshLv 47 years ago
Aren't you supposed to figure that out under the Microscope? If you are going to cheat you should just look it up don't ask us to.