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I need help with this lab question!?
Explain how internal standard calibration methods can be used to compensate for injector variability in GC (gas chromatography) analysis.
NOTE: I just guessed that it's because we are still able to get the correct concentration for the sample even though a serious problem was encountered in the sample injection process BUT I have a feeling this is incorrect or something.
1 Answer
- Simonizer1218Lv 75 years ago
Well, you are correct, but you don't explain the "how". The reason you can still get correct concentration determinations of the sample is because once you add an internal standard (known amount), whatever problems are encountered with the sample are also encountered for the standard, as it is now "part of" of the sample. So, once you determine % recovery of the internal standard, you can now apply that correction factor the recovery of the sample and calculate the correct amount.
