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Western blot protocol?
Can you please explain to me in detail all the steps for a western blot from preparing the samples, making the gel, transferring the gel, adding the primary and secondary antibodies, and developing in the dark room please.
Thank you!
1 Answer
- SmegheadLv 72 years ago
Yeah, sure! I'll be happy to spend a few hours typing up those thirty or so pages of technical detail for you, guessing at 90% of it, since I have no idea at all what the hell you're trying to do! After all, it's not as if Google exists or anything!!
