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Emily C
Kinetics help: shouldn't be too hard?
1. Sheep liver pyruvate carboxylase reacts with cyanate with subsequent loss of its acetyl-CoA dependent activity. The kinetics of the reaction were studied by isolating the effect of the enzyme by
running the reaction in an excess of cyanate. In fact, several sets of experiment were run with different concentrations of cyanate and an initial 1 μM concentration of the enzyme. The results were reported as the reduction in the initial activity for a given cyanate concentration. They are shown in the table here. The first column is time in seconds. The next four are all percent of initial activity for the following concentration (all mM) in order: 50, 100, 200, 300.
0 100 100 100 100
600 97 94 89 84
1200 94 89 79 71
2400 89 79 63 50
3600 84 71 50 35
4800 79 63 40 25
6000 75 56 31 18
9000 64 42 18 7.5
12000 56 31 10 3
A) Assume that the stoichiometry of the carboxylase-cyanate reaction is 1:1 and determine the percent change in composition of the least concentrated cyanate solution as the reaction proceeds from 0 to 12000 sec.
This is not supposed to be hard. I don't know what I'm missing.
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