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What is difference between promoter and primer?
I'm coming across both words on this virus chapter and my previous knowledge on both of them was only that they initiate the synthesis of new strand. What are their roles in eukaryortic cells?
2 Answers
- dooberheimLv 61 decade agoFavorite Answer
A promoter is a genetic element that an RNA polymerase binds to to initiate transcription of a downstream gene. Without a suitable promoter, the gene is not transcribed, and therefore is not expressed.
A primer is a short strand of DNA (called an oligonucleotide) that is complementary to a sequence that a scientist might want to replicate artificially. It acts as a start for a DNA polymerase (like Taq polymerase) to extend the sequence. This can be used to amplfiy a particular sequence by the polymerase chain reaction, or to incorporate labeled bases for a sequencing reaction.
DK
- Anonymous1 decade ago
I Quote:
- A primer is a nucleic acid strand, or a related molecule that serves as a starting point for DNA replication.
- a promoter is a regulatory region of DNA located upstream (towards the 5' region) of a gene, providing a control point for regulated gene transcription.
Source(s): http://en.wikipedia.org/
