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Decolorizing Agent in Gram Stain?
Is it really important to use 95% alcohol or can I use 70% to 90% with similar to duplicate results?
3 Answers
- saffronesqueLv 710 years agoFavorite Answer
Decolorization will take longer to occur with a more dilute solution. If you have a good supply of gram positive and gram negative bacteria, you could do a series of slides and increase the length of exposure to the more dilute alcohol solution until you get good decolorization of the gram negative smears. Some procedures uses an acetone-alcohol solution in place of 95% ethanol.
Source(s): Microbiology instructor - brodericLv 44 years ago
Gram-unfavorable bacteria have peptidoglycan interior the outer layer of their cellular wall, and gram-effective bacteria have peptidoglycan of their cellular wall a number of layers down. The stain reacts with the peptidoglycan.
- Anonymous10 years ago
i always used isotropically
