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Spectrophotometric Assay Question?
There's a blank on on of my university slides and I cannot work out the answer or how to find said answer beyond checking a textbook however I am not in town for my university library so I don't have access to the book.
The slides are on enzyme assays which I'm currently making notes on.
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EXAMPLE -
The hydrolase -glucosidase can be measured using artificial substrate.
p-nitrophenyl-(ALPHA)-D-glucose --> ?
Would the absorbance increase or decrease with time ?
Why?
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I'm sure I could draw conclusions if I understood what was meant by "p-nitrophenyl-(ALPHA)-D-glucose --> ?" but I don't understand...
as p-nitrophenol absorbs at 404 nm
p-nitrophenyl-X p-nitrophenol + X
1 Answer
- hcbiochemLv 77 years agoFavorite Answer
The enzyme hydrolyzes the linkage between glucose and p-nitrophenol, forming those two products. Since you form p-nitrophenol, the absorbance will increase over time.
