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if you are attemtpting to isolate a single colony from a mixed culture from a streak plate, how can you be ...
sure that the colony you chose to isolate is a pure culture?
haha! yes! i am the hitler of bacteria!!
4 Answers
- qetzalLv 41 decade agoFavorite Answer
If you really want to be sure, pick the colony and immediately streak it onto a fresh plate. Then take a single, well-isolated and well-formed colony from that plate. Even if the original colony wasn't pure, individual coloniew from the second plate should be. (You can even go through another round if you have to.)
Source(s): Lots of cloning experience in & after grad school - dooberheimLv 61 decade ago
Technically, you can't, but if your colonies are well separated enough, it is quite likely that the colony you pick came from one bacterium. To be sure, it is good to grow the culture, and streak it out again to make sure all the colonies look the same, or react as they should on various media.
DK
- Anonymous5 years ago
Pick a colony, disperse it in a drop of saline (as you might do preparing a Gram stain), then streak a droplet with the loop.
